AK Taq DNA Polymerase V2

AK Taq DNA Polymerase V2 is a genetically engineered variant of Taq DNA Polymerase with enhanced binding ability to DNA substrates through amino acid mutation,  and is more resistant to interference from impurities. It has the ability to amplify strands that conventional wild-type Taq cannot. Moreover, it retains the 5‘→3’ exonuclease activity of Taq polymerase, making it suitable for probe-based fluorescence quantitative PCR detection. The modification by dual Taq monoclonal antibodies enhances the inhibiting effects on the various active sites and improves the specificity. Prior to heating, the binding of the Taq monoclonal antibody at the anti Tag 5‘-3’ DNA polymerase active site to the Taq polymerase inhibits the polymerase activity and prevents nonspecific amplification at lower temperatures caused by non-specific annealing of primers or primer-dimer formation. The monoclonal antibody at Anti-Taq 5‘-3’ exonuclease active site binding to the Taq polymerase reduces exonuclease activity and prevents the degradation of primers and probes, which ensures the long-term stability of pre-mix products. When amplification reactions are heated to 95°C for two and half minutes, polymerase activity is restored due to the denaturation of the Anti-Taq monoclonal antibodies, yielding active polymerase that can be used directly under standard PCR reaction conditions.

In combination with optimized buffer, PCR proceeds rapidly and effectively with superior sensitivity, specificity, and product yield. 

In addition, the product exhibits decreased inhibition when in the presence of blood, soil, plant material, sputum, and other common PCR inhibitors and can be used for rapid PCR amplification protocols.

Composition