Fapon Biotech has developed a proprietary protein-free blocker to minimize non-specific interactions in an IVD assay. It would be of great utility to IVD developers.
FP MAP：General structure and mechanism of blocking
FP MAP, comprising an amphoteric polymer, has hydrophobic and hydrophilic sides in its chemical structure. The hydrophobic side binds to the non-specific protein binding sites on the solid substrate, while the hydrophilic side is exposed to the environment to build a protective hydrophilic layer that prevents the binding of interfering proteins.
Advantage
over conventional blockers
Non-specific binding test
FP MAP's performance in ELISA:
FP MAP is the best in inhibiting non-specific binding on ELISA platform.
FP MAP's performance in CLIA:
FP MAP is the best in inhibiting non-specific binding on CLIA platform.
Sensitivity test
FP MAP as a blocking reagent for magnetic bead
FP MAP showed the best performance in the sensitivity tests in comparison to other commercial blockers
FP MAP as a blocking additive in magnetic bead solution or AE solution
FP MAP can critically increase HIV immunoassay sensitivity in comparison to other commercial blockers
Avoiding cross-reactivity
FP MAP as a blocking additive in bead solution or AE solution
Stability test
FP MAP is added as a blocking additive in AE, magnetic bead solution, or both of the above. The reagents are heated at 37°C for 7 days, RLUs are measured and compared to the control without FP MAP and the deviations are calculated.
