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Protein expression platform
We have four powerful protein expression systems: mammalian cells, insects, yeasts and bacteria.
Mamnesis, our mammalian expression system, can be used to produce mammalian proteins that have the most native structure and activity due to its physiologically relevant environment. By optimizing cell strains and expression vectors, the protein expression level is four times higher than commercial expression systems.
InsectBac, the insect–baculovirus expression system, enables high levels of post-translational processing and high-density culture, making it an ideal choice for the expression of complex proteins and viral proteins.
Transmembrane proteins hardly retain their native conformations in soluble form in vitro due to the hydrophobic nature of the multiple membrane-spanning segments. Expression level of transmembrane proteins is usually very low. With the virus-like particle (VLP) technology, we can overcome these challenges and obtain transmembrane proteins with high activity in high yields.
Purification and large-scale fermentation guarantee lot-to-lot consistency as well. With a rich toolkit of technologies, we can produce quality antigens in large quantities at low costs.
Mamnesis
Mamnesis is our in-house technology for transient transfection and stable cell strain development for protein expression. Integration of the gene of interest (GOI) into host cells is as efficient as 95% and above. The Protein expression level is four times higher than common commercial expression systems.
InsectBac
InsectBac is an insect–baculovirus expression system that combines bacmid engineering and expression system optimization. Protein expression level and quality are four times higher than common commercial expression systems.
Prokaryotic expression system
We can choose from a selection of expression vectors and host bacteria to efficiently develop and optimize the host strains. To improve protein yields and molecular folding, we develop genetically engineered strains, screen for suitable mutants, and optimize metabolic pathways, among others. After we obtain the recombinant proteins of interest, we carry out qualitative and quantitative analysis of molecular weight, purity and functions using technologies such as SDS-PAGE, western blotting and mass spectrometry.
Yeast expression system
Compared to bacteria, yeasts have been a popular choice as being a fast and cost-effective method to produce recombinant proteins in large quantities, particularly those requiring complex post-translational modifications with well-characterized genetics such as glycosylation and phosphorylation. With our yeast protein expression technologies, we can develop and optimize yeast culture processes, and control culture conditions in bioreactors such as dissolved oxygen level, pH, temperature and nutrient supply. This opens up possibilities of high-density culture and excellent protein yields.
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