Overview
Based on GRASPlex® pathogen-targeted amplicon sequencing (tNGS) and 16S amplicon sequencing technologies, the vaginal micro-ecological health assessment product comprehensively evaluates women's vaginal health by detecting 73 core and common target microorganisms in Chinese women, helping women to detect potential microbial imbalances early and preventing the occurrence of diseases. At the same time, it can assist clinical diagnosis and treatment to quickly identify the cause of disease and avoid antibiotic abuse and over-treatment. It provides scientific guidance on vaginal care, probiotic regimen and precise diagnosis and treatment.
Test content
Significance
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Health warning
Through a comprehensive understanding of the composition of vaginal microorganisms and the type of flora structure, early detection of potential microbial imbalances and infectious pathogens, thus suggesting and early warning of vaginal health, and providing scientific guidance and recommendations for the prevention of the development of diseases.
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Accurate diagnosis and treatment
To assist clinical diagnosis and treatment, quickly identify the cause of the disease, and avoid the abuse of antibiotics and over-treatment. Provide scientific basis for the change from traditional single antibacterial treatment to the new treatment of antibacterial-mucosal repair-restoration of microecology mediated by probiotics and other auxiliary microecological agents.
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Scientific intervention
Based on the type of flora structure, the probiotic regimen and other interventions can be formulated more scientifically and accurately.
Performance Parameter
Sample type | vaginal swab |
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Sample concentration (dsDNA Qubit) | >0.1ng/ul |
Starting amount | >>3ng (volume: 30ul) |
library construction process | 2-step PCR |
Applicable platforms | Illumina,MGI |
Target area | Conserved specific sequences of microorganisms + 16S V4 |
Number of amplicons (excluding 16S and internal and external parameters) | 134 |
Number of pools | 1 |
Amplicon lengths | 200-300bp(average 238bp) |
Detection limit | >=500copies/ml |
Depot construction cycle | 4.5 hours |
man-hour | <1 hour (library building) |
Sequencing type | PE150 |
Sequencing data volume | Recommended >= 1M reads |
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